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Chromolith HPLC

Chromolith® HPLC Columns

Speed and performance based on revolutionary monolithic silica technology

In contrast to conventional particle-packed columns, monolithic silica columns are made of a continuous piece of porous silica, utilizing a sol-gel process leading to rod columns that possess a defined bimodal pore structure with macro- and mesopores in the micro- and nanometer range. High permeability and porosity of the silica skeleton and the resulting low back-pressure allow for more flexible flow rates compared to particulate columns and enable high-throughput analysis without loss of separation efficiency and peak capacity.

 

This revolutionary bimodal pore structure provides a unique combination of macropores and mesopores.

Chromolith® HPLC columns

Mesopores 13nm
The mesopores form the fine porous structure (average pore size 13 nm) and create the large uniform surface area on which adsorption takes place, thereby enabling high-performance chromatographic separation.
Macropores 2µm
The macropores allow rapid flow of the mobile phase at low pressure. Their average size is 2 μm.

 

Characterization of Chromolith® HPLC columns
The use of HPLC columns containing the classic 3 or 5 μm small silica particles often results in high back-pressure.

This high back-pressure may damage both the column and the HPLC system; therefore, classic HPLC columns have limited length and a limited number of theoretical plates. Attempts have been made to increase the plate count by decreasing the particle size, but this results in unacceptable back-pressure and limits the variety of separations that can be satisfactorily achieved.

Many of today’s scientists wish to speed up the entire separation process and therefore acceleration of the analysis processes has become one of the most important issues in high-performance liquid chromatography. Laboratory automation of HPLC systems has come a long way toward improving sample throughput by enabling around-the-clock operation. Chromolith® HPLC columns provide excellent separations in a fraction of the time that a standard particulate column takes, because they are made from highly porous monolithic rods of silica with a revolutionary bimodal pore structure.

 

 

 

 

 

 

Chromolith® columns at glance

  Column length [mm] Chromolith® HPLC column 
Guard / Trap
column 5 mm
Guard / Trap column 10 mm 25 mm 50 mm 100 mm

 
25 mm   Chromolith® RP-18 endcapped

Chromolith® Si (silica)
    Chromolith® RP-18 endcapped

Chromolith® Si (silica)
10 mm   Chromolith® RP-18 endcapped

Chromolith® Si (silica)
    Chromolith® RP-18 endcapped

Chromolith® Si (silica)
4.6 mm Chromolith®
RP-18 endcapped


Chromolith®
RP-18 endcapped HighResolution


Chromolith® NH2
Chromolith® RP-18 endcapped Chromolith®
RP-18 endcapped


Chromolith®
RP-18 endcapped
HighResolution


Chromolith® NH2
Chromolith®
RP-18 endcapped


Chromolith®
RP-18 endcapped HighResolution


Chromolith® NH2
Chromolith®
RP-8 endcapped


Chromolith®
RP-18 endcapped


Chromolith®
RP-18 endcapped HighResolution


Chromolith® Si (silica)

Chromolith® NH2
3 mm Chromolith® RP-18 endcapped   Chromolith®
RP-18 endcapped
Chromolith®
RP-18 endcapped
Chromolith®
RP-18 endcapped
2 mm Chromolith® RP-18 endcapped   Chromolith®
RP-18 endcapped
Chromolith®
RP-18 endcapped
Chromolith®
RP-18 endcapped
Chromolith® HPLC column  

 

Chromolith® various column lenghts

Three different column lengths of Chromolith® columns are available: the Chromolith® Flash, the Chromolith®
SpeedROD/FastGradient and the Chromolith® Performance columns, which are opening the door to high-speed separations!

Chromolith® 25 mm length – for ultra-fast separation of simple mixtures
Chromolith® Flash columns are very short and perfect for ultra-fast analysis simple mixtures.
The length of the column is 25 mm and therefore the number of theoretical plates of the Chromolith® Flash column is sufficient for easy separations. The major focus of the Chromolith® Flash columns is clearly on the speed of analysis, since it provides the chromatographer with the fastest HPLC column available on the market!

Chromolith® 50 mm length – for fast separation of simple mixtures
Chromolith® SpeedROD columns are short and perfect for fast analysis.
Chromolith® SpeedROD HPLC columns are ideal for use in the rapid screening of samples especially for in-process control as well as in research laboratories or those specializing in organic synthesis, e.g. combinatorial chemistry.

Chromolith® 100 mm length – for rapid separation of more complex mixtures
Chromolith® Performance columns provide rapid high-quality separation of complex multi-component mixtures. They are therefore perfect for use as a routine analytical tool in the quality control laboratory or in research laboratories where more complex mixtures are being analyzed.

Chromolith® Performance RP-18 endcapped 100-4.6 mm validation kit
For correct method validation, it is essential to assess all possible sources of variations. To assist the validation process, the Chromolith® validation kit includes three columns from three different production batches, in order to compare the batch-to-batch reproducibility and quality. The Chromolith® Performance RP-18 endcapped validation kit is therefore perfect for use as an appropriate tool in quality control laboratories or in validation laboratories.
The cost and time savings made possible by the use of Chromolith® columns can repay the expense of a method revalidation within just one month.

 

Differentiation between Chromolith® RP-18 endcapped and Chromolith® RP-18 endcapped HighResolution

Differentiation between Chromolith® and Chromolith® HighResolution

The new Chromolith® HighResolution has at least 50% higher efficiency, excellent peak symmetry and yet a more than 30% longer lifetime compared with particulate columns. Two Chromolith® HighResolution columns can easily be coupled in order to achieve even higher resolution. A polar end-capped stationary phase enables the elution of basic compounds with no peak tailing.
Matrix-rich samples should be analyzed with Chromolith® as this type of column has a longer lifetime. In addition, a lower back-pressure enables the coupling of even more columns if necessary.

 

 

Further suggested reading 

Chromolith® HPLC  
  Detailed information about Chromolith®
HPLC columns
 
  Chromolith® CapRod®  
  Chromolith® column coupler  
  Chromolith® HPLC FastGradient  
  Chromolith® HPLC guard cartridges  
  Chromolith® NH2  
  Chromolith® Prep  
  Chromolith® RP-8 endcapped  
  Chromolith® RP-18 endcapped  
  Chromolith® RP-18 endcapped HighResolution  
  Chromolith® Si  
  Chromolith® SemiPrep  

© Merck KGaA, Darmstadt, Germany, 2014


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