Chromolith® RP-18 endcapped HighResolution
The faster way to trouble-free high resolution separations
Revolutionary monolithic silica columns are a single rod of high purity monolithic silica instead of particles. A unique bimodal pore structure gives greatly improved chromatographic performance. This leads mainly to improved separation efficiency and column back-pressure and allows for faster flow rates, considerably reducing the analysis time while enhancing the lifetime.
Benefits of the new Chromolith® HighResolution
- Column performance corresponds to sub 3 µm particle-packed columns and is at least 50% higher compared with our standard Chromolith® columns
- Back-pressure at least half that of particular columns
- Minimum 30% longer column lifetime compared with particle-packed columns
The new Chromolith® HighResolution columns offer higher efficiency and improved peak shape combined with lower back-pressure which is more than two times lower than any particulate packed column with the same dimensions.
Differentiation between Chromolith® and Chromolith® HighResolution
The new Chromolith® HighResolution has at least 50% higher efficiency, excellent peak symmetry and still a more than 30% longer lifetime compared with particulate columns. Two Chromolith® HighResolution columns can easily be coupled in order to achieve even higher resolution. A endcapped stationary phase enables the elution of basic compounds with no peak tailing.
Matrix-rich samples should be analysed with Chromolith® as this type of column will have a longer lifetime. In addition, a lower back-pressure would allow coupling of even more columns if necessary.
|Chromolith® Performance |
RP-18e, 100-4.6 nm
RP-18e, 100-4.6 nm
|Mobile phase||Acetonitrile/water 60/40|
|Flow rate||2.0 ml/min|
|Detection||UV 254 nm|
|Inj. volume||5 µl|
Specification of Chromolith® HighResolution RP-18 endcapped
|Macropore size||1.15 micrometer|
|Mesopore size||15 nm (150 Angström)|
|Pore volume||1 mL/g|
|Total pore volume||2.9 mL/g|
|Surface area||250 m2/g|
|Surface modification||RP-18 endcapped|
|Carbon content||18 %|
Method transfer from particulate to monolithic Chromolith® columns
Method transfer from particulate to monolithic Chromolith® columns is made easy since the chromatographic selectivity of Chromolith® columns is very similar to that of many modern particulate HPLC columns (e.g. Purospher®). Chromolith® columns are now included in the US Pharmacopoeia in categories L1, L3 and L7.
Further suggested reading