Neisser's Solutions - Ready-to-use Staining Solution for Corynebacterium diphtheriae
|Increasing figures for infectious diseases are bring more attention to staining solutions and staining kits worldwide. In those diagnostic areas, where staining is an option, it is used as the first test for a lot of cases.
Bacteriological staining gives fast results and can indicate the next diagnostic step. In the non-specific Neisser procedure, methylene blue , crystal violet and chrysoidine are used for the detection of metachromatic granules, or so-called Babes-Ernst polar bodies, especially in diphtheria bacteria. With a defined pH value, methylene blue and crystal violet will be bound in the polar bodies or structures (Volutin bodies), but not bound in the rest of the bacterial cell. The polar bodies become visible as dark dots. In the counter stain procedure, the body of the bacterium is stained by chrysoidine but this is only partly absorbed by the polar bodies.
Neisser solution can be carried out with various specimens such as air-dried, heat-fixed smears of bacteriological material, e.g. sputum, FNAB, lavages, imprints, body fluids, pus, exudates, liquid and solid cultures.
The 3 Neisser solutions from Merck are ready-to-use and Neisser solution Ia and Ib are mixed immediately prior to use in a ratio 1:1, Neisser solution II is used for the counter stain.
This established staining protocol gives good and reproducible results:
- Stain for 1 min in methylene blue-crystal violet solution
- Wash very briefly - or not at all - with water
- Counter stain immediately with Neisser's solution II for 10 sec
- Wash briefly with water and dry between sheets of blotting pape
- Polar bodies dark brown to black
- Bacterium body brownish
Ready-to use stain solutions can visibly improve the quality of stains used routinely.
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