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Merck Chemicals Ltd.
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30 mai 2012
Chargement
Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
| Informations produit | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein | ||
| Clone | 8G8 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.30. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Oui | ||
| Positive control | MCF-7 cells, Human lymphoma tissue, HeLa cells | ||
| Negative control | 293T cells | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Données | |||
|---|---|---|---|
 Detection of human ENO1 by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1-5 µg/ml). Detection: chmiluminescence.  Detection of human ENO1 by Immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (10 µg/ml). Detection: fluorescence.  Detection of human ENO1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human lymphoma tissue. Primary antibody: Anti-ENO1 Mouse mAb (8G8) (Cat. No. DR1061) (1 µg/ml). Detection: colorimetric (DAB). |
