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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
Recognizes the ~60 kDa GBA protein in MCF-7 and HeLa cells and human breast cancer tissue.
| Informations produit | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids of 146-236 human GBA, expressed as a GST fusion protein | ||
| Clone | 2E2 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Oui | ||
| Positive control | MCF-7 cells, HeLa cells, Human breast cancer tissue | ||
| Negative control | 293T | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Données | |||
|---|---|---|---|
 Detection of human GBA by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human GBA by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (10 µg/ml). Detection: fluorescence.  Detection of human GBA by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-GBA Mouse mAb (2E2) (Cat. No. AP1140) (3 µg/ml). Detection: colorimetric (DAB). |
