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Merck Chemicals Ltd.
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United Kingdom
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e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
Recognizes the ~140 kDa MYLK protein in human stomach tissue.
| Informations produit | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | Full-length, human MYLK (aa 1-992) | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
|
||
| Avoid freeze/thaw | Oui | ||
| Positive control | Human stomach tissue | ||
| Negative control | 293T cells | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
||
| Données | |||
|---|---|---|---|
 Detection of human MYLK by immunoblotting. Sample: Whole tissue extract from human stomach. Primary antibody: Anti-MYLK Rabbit pAb (t. No. ST1657) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human MYLK by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-MYLK Rabbit pAb (t. No. ST1657) (1:1200) and an Anti-CTTN mouse mAb (1:50). Detection: fluorescence using Duolink<sup>®</sup> 30 Detection Kit 613 (Red) and DAPI (blue) counterstain. |
