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Merck Chemicals Ltd.
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NG9 2JR Nottingham
United Kingdom
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30 mai 2012
Chargement
A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers.
One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Applications |
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| Avoid freeze/thaw | Oui | ||
| Sensitivity | < 1 ng/ml | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture supernatant, sera, or plasma | ||
| Kit contains | 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
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Traitement des matériaux dangereux : En raison de la nature dangereuse des matériaux contenus dans cet envoi, des frais d'expédition supplémentaires pourront être appliqués à votre commande. Certaines tailles peuvent être exonérées des frais d'expédition supplémentaires pour matériaux dangereux. Veuillez contacter la filiale la plus proche pour de plus amples informations sur ces frais. |
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| Catégorie de stockage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Informations de sécurité | |||
|---|---|---|---|
| Phrase S |
S: 26-36/37/39-45 |
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| Phrase R |
R: 23/24/25-35-36/37/38-40-43 |
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| Données | |||
|---|---|---|---|
 Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).  The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.  Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).  Dilution recovery using different cell lines. |
