CBA025
InnoCyte™ ECM Cell Adhesion Assay, Laminin/Basement Membrane Complex (BMC)
Pour les questions d'ordre général, veuillez contacter notre Service Clients :
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.
• Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
• Sensitive: cell-permeable fluorescent dye; no cell permeabilization needed
• Easy to use: does not require tedious staining method or expensive complicated equipment
• Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
• Sensitive: cell-permeable fluorescent dye; no cell permeabilization needed
• Easy to use: does not require tedious staining method or expensive complicated equipment
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 72 Tests (36 Laminin and 36 BMC) | ||
| Detection method | Fluorometric | ||
| Protect from Light | Oui | ||
| Avoid freeze/thaw | Oui | ||
| Assay time | 2.5 h | ||
| Sample type | Adherent cultured cells | ||
| Kit contains | Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Données | |||
|---|---|---|---|
 ~40,000 cells were added to wells containing laminin I, BMC, or BSA and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO<sub>2</sub>. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO<sub>2</sub>. Fluorescence was measured as indicated in the Detailed Protocol. HT-1080 cells displayed appreciable binding to poly-L-lysine and served as a positive control (data not shown). |
