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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
The InnoCyte™ Laminin-Based 96-well Cell Invasion Assay is designed to determine the invasive capacity of cells using laminin as a significant barrier to invasion or to screen potential anti-metastatic agents in vitro in a convenient, high-throughput, 96-well format. The 8 μm pore size is appropriate for all types of adherent cells such as epithelial, endothelial, or fibroblast cells. The assay is based on the Boyden-chamber principle. Each well of the 96-well cell culture insert contains a polycarbonate membrane with an 8 μm pore size that is coated with a laminin protein layer. The laminin layer forms an effective barrier that prevents non-invasive cells from passing through the 8 μm pores. Invasive cells that can degrade the laminin layer will migrate through the membrane and attach to the underside of the membrane. The invasive cells are dislodged from the underside of the cell culture insert and stained with a fluorescent dye in a single step. Fluorescence is determined using a fluorimeter with an excitation wavelength of ~485 nm and an emission wavelength of ~520 nm.
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Oui | ||
| Avoid freeze/thaw | Oui | ||
| Assay time | 14-26 h | ||
| Sample type | Cells | ||
| Kit contains | 96-Well Laminin-Coated 96-Well Cell Invasion Chamber, Black 96-Well Plate, Additional Tray , Calcein-AM Solution, Cell Detachment Buffer, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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