CBA042
InnoZyme™ TACE Activity Kit
α-Secretase Activity Kit, TNF-α Converting Enzyme Activity Kit, ADAM17 Activity Kit
Pour les questions d'ordre général, veuillez contacter notre Service Clients :
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
The InnoZyme™ TACE Activity Kit is a specific and sensitive assay designed to measure human TACE activity in cell lysates and biological samples and for screening enzyme inhibitors. An Anti-Human TACE-Coated 96-Well Plate is pre-coated with a monoclonal antibody specific for human TACE that captures the enzyme. Unbound material is discarded, the plate is washed, and the activity of captured TACE is measured using an internally quenched fluorescent substrate, MCA-KPLGL-Dpa-AR-NH2. Cleavage of the scissile amide bond, G-L, releases the fluorophore from the quenching molecule, Dpa, resulting in an increase in fluorescence. Fluorescence of the cleaved product, MCA-KPLG, is measured at an excitation wavelength of ~324 nm and emission wavelength of ~400 nm. The level of fluorescence is directly related to the enzyme activity.
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
• Highly specific: based on immunocapture of human TACE
• Highly sensitive and quantitative: assay range 5-100 ng/ml
• Convenient: 96-well format and non-radioactive detection
• Versatile: appropriate for measuring active TACE and high-throughput screening of TACE inhibitors
| Informations produit | ||||
|---|---|---|---|---|
| Format | 96-well plate | |||
| Form | 96 Tests | |||
| Detection method | Fluorometric | |||
| Protect from Light | Oui | |||
| Avoid freeze/thaw | Oui | |||
| Components |
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| Assay range | 5-100 ng/ml as measured with purified recombinant human TACE | |||
| Sample type | Cells | |||
| Positive control | Human recombinant TACE | |||
| Kit contains | Anti-Human TACE-Coated 96-Well Plate, Control, Substrate, Sample Buffer, Assay Buffer, Wash Buffer, Plate Sealer, and a user protocol. | |||
| Declaration | Not available for sale in Germany. | |||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Données | |||
|---|---|---|---|
 The activity of increasing concentrations human recombinant TACE (Cat. No. PF133) was measured according to the Detailed Protocol above.  Human colorectal adenocarcinoma cells, DLD1 and HT-29 and human glioblastoma cells, T98G, were cultured in DMEM medium supplemented with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) and the manufacturer's recommended protocol. TACE activity was measured according to the Detailed Protocol above. RFU is reported per mg protein. |
