Pour les questions d'ordre général, veuillez contacter notre Service Clients :
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Téléphone : +44 (0)115 9430840
Fax : +44 (0)115 9430951
e-mail : customer.service@merckbio.eu
30 mai 2012
Chargement
Merci de bien vouloir contacter notre Service Clients pour commander ce produit.
This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of captured nucleosomes and is detected following incubation with streptavidin-HRP conjugate. HRP catalyzes the conversion of colorless tetramethylbenzidine to blue. The addition of stop solution changes the color to yellow, the intensity of which is proportional to the number of nucleosomes in the sample. By comparing the absorbance of unknown with that obtained from the standards, one can assign a nucleosome unit value to the unknown sample. Can be used to assign apoptotic index.
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | a broad range of species | ||
| Applications |
|
||
| Protect from Moisture | Oui | ||
| Avoid freeze/thaw | Oui | ||
| Unit definition | One unit (U) is defined as the amount of nucleosomes from 444 UV-treated Daudi cells. | ||
| Sensitivity | 0.15 U/ml | ||
| Assay range | 00 - 12 U/ml | ||
| Assay time | 5 h | ||
| Sample type | Cell lysates | ||
| Kit contains | 96-Well Plate (12 removable strips) Coated with a DNA Binding Protein, Nucleosome Standard, Lysis Buffer/Sample Diluent, Biotinylated Anti-Histone 3 Antibody, 400X HRP-Streptavidin Conjugate, Conjugate Diluent, Chromogenic Substrate, 20X Concentrated Wash Solution, Stop Solution, 4 Plate Sealers, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
|
Traitement des matériaux dangereux : En raison de la nature dangereuse des matériaux contenus dans cet envoi, des frais d'expédition supplémentaires pourront être appliqués à votre commande. Certaines tailles peuvent être exonérées des frais d'expédition supplémentaires pour matériaux dangereux. Veuillez contacter la filiale la plus proche pour de plus amples informations sur ces frais. |
|||
| Catégorie de stockage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Informations de sécurité | |||
|---|---|---|---|
| Phrase S |
S: 26-36/37/39-45 |
||
| Phrase R |
R: 23/24/25-35-36/37/38-40-43 |
||
