CBA006
PhosphoDetect™ ERK1/2 (pThr¹85/pTyr¹87) ELISA Kit
p44 MAP Kinase, Phospho-Specific (Thr¹85/Tyr¹87) ELISA
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Merck Chemicals Ltd.
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NG9 2JR Nottingham
United Kingdom
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30 mai 2012
Chargement
Detects the dual phosphorylated forms of ERK1 at Thr202 and Tyr204 and ERK2 at Thr185 and Tyr187. This activation occurs as a result of treatment with a large variety of stimuli including mitogens, cytokines, and growth factors. Although this kit was designed for human samples, it cross-reacts with mouse and rat.
| Alternatives | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| Produits alternatifs pour PhosphoDetect™ ERK1/2 (pThr¹85/pTyr¹87) ELISA Kit | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| Accessoires pour PhosphoDetect™ ERK1/2 (pThr¹85/pTyr¹87) ELISA Kit |
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | Oui | ||
| Sensitivity | ≤0.8 units/ml | ||
| Assay range | 1.6-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Kit contains | Coated 96-Well Plate, ERK1/2 Phospho-Thr¹85/Tyr¹87 Standard, Diluents, Detector Antibody, Secondary Antibody, Sample Treatment Buffer, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Informations de sécurité | |||
|---|---|---|---|
| Phrase S |
S: 26-36/37-45 |
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| Phrase R |
R: 21/22-36/38 |
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| Données | |||
|---|---|---|---|
 Jurkat cells were treated with 100 ng/ml PMA for 10 min and cell lysates were prepared. Cell lysate prepared with Cell Lysis Buffer was either boiled for 5 min, treated with Sample Treatment Buffer, or not treated. Cell lysate was also prepared using Denaturing Cell Lysis Buffer. Samples were analyzed with the ERK1/2 and the PhosphoDetect™ ERK (Thr<Sup>185</sup>/Tyr<sup>187</sup>) ELISA Kits.  The sensitivity of this ELISA was compared to immunoblotting using known quantities of ERK1/2 Phospho-Thr<sup>185</sup>/Tyr<sup>187</sup>. The data shows that the sensitivity of the ELISA is approximately 4X greater than that of immunoblotting. The bands shown in the immunoblotting were developed using rabbit anti-ERK1/2 Phospho-Thr<sup>185</sup>/Tyr<sup>187</sup> and an alkaline phosphatase conjugated anti-rabbit IgG followed by chemiluminescent detection.  Natural ERK1/2 Phospho-Thr<sup>185</sup>/Tyr<sup>187</sup> from PMA-treated Jurkat cell lysates was serially diluted in Standard Diluent Buffer. The absorbance of each dilution was plotted against the ERK1/2 Phospho-Thr<sup>185</sup>/Tyr<sup>187</sup> standard curve. Parallelism is demonstrated in the figure above and indicates that the standard accurately reflects natural ERK1/2 Phospho-Thr<sup>185</sup>/Tyr<sup>187</sup> content in samples. |
