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Merck Chemicals Ltd.
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NG9 2JR Nottingham
United Kingdom
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30 mai 2012
Chargement
Detects and quantifies the level of PRAS40 (Proline-Rich AKT Substrate of 40 kDa) independent of its phosphorylation state. PRAS40 is a 14-3-3 binding protein that is a direct substrate of Akt. It is believed to influence protein interactions, nuclear transport, and enzyme activities.
| Informations produit | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Sensitivity | <200 pg/ml | ||
| Assay range | 0.313-20 ng/ml | ||
| Assay time | 4 h | ||
| Kit contains | PRAS40 Standard, Standard Diluent Buffer, PRAS40 Antibody-Coated 96-Well Plate, Rabbit Anti-PRAS40 Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers, and a user protocol. | ||
| Information relative au stockage et expédition | |||
|---|---|---|---|
| Catégorie de stockage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
||
| Données | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of PRAS40. The data show that the sensitivity of the ELISA is approximately 2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-PRAS40 antibody and chemiluminescent detection. |
