124007
Akt Activity Immunoassay Kit
Protein Kinase B Activity Assay Kit
Wenn Sie allgemeine Fragen haben, wenden Sie sich bitte an unseren Kundenservice:
Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-Mail: customer.service@merckbio.eu
30 Mai 2012
wird geladen
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
Note: 1 kit = 40 assays.
| Produktinformationen | |||
|---|---|---|---|
| Format | Immunoblot | ||
| Form | 40 Tests | ||
| Detection method | Immunoblot | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | Ja | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates, tissue extracts | ||
| Kit contains | Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3α Protein/ATP Mixture, Kinase Assay Buffer, GSK-3α, Phospho-Specific Antibody, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
