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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
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E-Mail: customer.service@merckbio.eu
30 Mai 2012
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A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers.
One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Applications |
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| Avoid freeze/thaw | Ja | ||
| Sensitivity | < 1 ng/ml | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture supernatant, sera, or plasma | ||
| Kit contains | 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
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Achtung Gefahrgut:Aufgrund der Natur des in dieser Lieferung enthaltenen Gefahrgutes können zusätzliche Versandkosten für Ihren Auftrag entstehen. Bestimmte Größen können ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen sein. Bitte kontaktieren Sie Ihr lokales Verkaufsbüro für weitere Informationen bzgl. dieser Gebühren. |
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| Lagerkategorie | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 26-36/37/39-45 |
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| R-Satz |
R: 23/24/25-35-36/37/38-40-43 |
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| Daten | |||
|---|---|---|---|
 Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).  The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.  Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).  Dilution recovery using different cell lines. |
