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Merck Chemicals Ltd.
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30 Mai 2012
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This kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on detection of the cleavage of the substrate YVAD-AFC. Upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (~505 nm), which can be measured using a fluorometer or a fluorescence microplate reader.
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 100 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Ja | ||
| Assay time | 3-3.5 h | ||
| Sample type | cell lysates | ||
| Kit contains | Cell Lysis Buffer, Reaction Buffer, YVAD-AFC Substrate, DTT, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
||
| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 26-36-39-61 |
||
| R-Satz |
R: 22-36/38-41-52/53 |
||
