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Merck Chemicals Ltd.
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NG9 2JR Nottingham
United Kingdom
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30 Mai 2012
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A highly sensitive and selective fluorogenic assay for the detection of Cathepsin L from human and mouse samples. Cathepsin L is detected with the fluorogenic substrate Z-Phe-Arg-AMC (Exc. max: 360 nm; Emi. max: 460 nm). Interference from Cathepsin B is eliminated by the incorporation of CA 074, a specific cathepsin B inhibitor. This kit is also useful for screening cathepsin L inhibitors.
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Ja | ||
| Avoid freeze/thaw | Ja | ||
| Sensitivity | 1.2 ng/ml | ||
| Assay range | 1.56-100 ng/ml | ||
| Assay time | 1.5 - 2 h | ||
| Sample type | Cell lysates, tissues, or purified cathepsin L | ||
| Positive control | Cathepsin L | ||
| Kit contains | Cathepsin L Substrate, AMC Calibration Standard, Cathepsin L Control, Cathepsin L Activator, CA 074, Cathepsin L Inhibitor, Assay Buffer, Sample Buffer, Diluent, Microwell Plate, Plate Sealer, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
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Achtung Gefahrgut:Aufgrund der Natur des in dieser Lieferung enthaltenen Gefahrgutes können zusätzliche Versandkosten für Ihren Auftrag entstehen. Bestimmte Größen können ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen sein. Bitte kontaktieren Sie Ihr lokales Verkaufsbüro für weitere Informationen bzgl. dieser Gebühren. |
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| Lagerkategorie | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Daten | |||
|---|---|---|---|
 Calibration curve generated using the AMC Calibration Standard included in the InnoZyme™ Cathepsin L Activity Kit. The assay was carried out as specified in the Cathepsin L Inhibitor Screening protocol. |
