CBA003
InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay kit, Fluorogenic
MMP-2/MMP-9 Activity Assay Kit, Fluorogenic
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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-Mail: customer.service@merckbio.eu
30 Mai 2012
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A sensitive fluorogenic assay (Exc. max: ~325 nm; Emi. max.: ~393 nm) for the measurement of gelatinases (MMP-2 and MMP-9). Also useful for the screening of gelatinase inhibitors.
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorogenic | ||
| Protect from Light | Ja | ||
| Avoid freeze/thaw | Ja | ||
| Sensitivity | 20 ng/ml | ||
| Assay time | 2 - 6 h | ||
| Sample type | Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme | ||
| Positive control | MMP-9 (Cat. No. PF140) | ||
| Kit contains | Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
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Achtung Gefahrgut:Aufgrund der Natur des in dieser Lieferung enthaltenen Gefahrgutes können zusätzliche Versandkosten für Ihren Auftrag entstehen. Bestimmte Größen können ggf. von den Versandzuschlägen für Gefahrgüter ausgenommen sein. Bitte kontaktieren Sie Ihr lokales Verkaufsbüro für weitere Informationen bzgl. dieser Gebühren. |
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| Lagerkategorie | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Daten | |||
|---|---|---|---|
 Human Pro-MMP-2 (supplied) was activated as outlined in the Reagent Preparation section above and assayed for activity as outlined in the Detailed Protocol, incubating for a total of 3 h.  Activated MMP-9 (Cat. No. PF140) was assayed without APMA at 37°C for 3 h according to the Detailed Protocol outlined above.  Serum from 5 different donors was diluted 1:5 in Activation Buffer and HT1080 culture supernatant was diluted 1:3 with Activation Buffer. All samples were incubated at 37°C for 3 h according to the Detailed Protocol outlined above. |
