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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-Mail: customer.service@merckbio.eu
31 Mai 2012
wird geladen
This product has been discontinued.
This product has been discontinued.
Detects and quantifies the levels of p38 MAPK protein independent of its phosphorylation state. p38 MAPK activation occurs in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, LPS, and UV light. Although this kit is designed for human samples, it cross-reacts with mouse and monkey.
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, monkey, mouse | ||
| Avoid freeze/thaw | Ja | ||
| Sensitivity | ≤16 pg/ml | ||
| Assay range | 31.2-2000 pg/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | Coated 96-Well Plate, p38 MAPK Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Daten | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of p38 MAPK. The data presented in Figure 1 show that the sensitivity of the ELISA is ~10x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-p38 MAPK antibody and, chemiluminescent detection. |
