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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
United Kingdom
Telefon: +44 (0)115 9430840
Fax: +44 (0)115 9430951
E-Mail: customer.service@merckbio.eu
31 Mai 2012
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A fluorometric assay kit for the measurement of Protein Tyrosine Phosphatase (PTP) activity. The assay uses a fluorogenic phosphopeptide substrate quenched internally by resonance energy transfer. The substrate contains a fluorophore (MCA) and a quencher group (DNP) as well as a phosphotyrosine residue located between these groups. Reaction of the substrate with PTP followed by treatment of the dephosphorylated substrate with chymotrypsin results in the cleavage of the peptide, which results in an increase in fluorescence.
| Produktinformationen | |||
|---|---|---|---|
| Format | 96-well format | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Ja | ||
| Sensitivity | 0.2-0.4 pmol | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | PTP Substrate, Assay Buffer, Chymotrypsin Solution, Control Peptide, Microtiter Plate, Stop Solution, and a user protocol. | ||
| Lager- und Versandinformationen | |||
|---|---|---|---|
| Lagerkategorie | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
||
| Sicherheitshinweise | |||
|---|---|---|---|
| S-Satz |
S: 23-24-26-36/37 |
||
| R-Satz |
R: 36/37/38-42 |
||
