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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
A quantitative colorimetric assay for the measurement of Bcl-2. Able to detect significant decreases in Bcl-2 protein levels before significant levels of apoptosis are detected. Kit has high sensitivity, a broad dynamic range, and contains all required buffers.
One unit of Bcl-2 equals the Bcl-2 activity in 5.6 X 104 HL-60 cells
| Informasi produk | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Applications |
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| Avoid freeze/thaw | Ya | ||
| Sensitivity | < 1 ng/ml | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture supernatant, sera, or plasma | ||
| Kit contains | 96 Well Coated Plate, Antigen Extraction Reagent, Lyophilized Bcl-2 Standard, Sample Diluent, Anti-FITC Conjugated Detector Antibody, Concentrated Anti-FITC Peroxidase Conjugate, Conjugate Diluent, Substrate, Wash Buffer, Stop Solution, Adhesive Plate Sealers, and a user protocol. | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
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Informasi Bahan Berbahaya: Karena sifat dari Bahan Berbahaya ini kami mengenakan biaya pengiriman tambahan yang dapat ditambahkan ke pesanan Anda. Kemasan tertentu mungkin saja tidak dikenakan tambahan biaya pengiriman bahan berbahaya. Silakan hubungi kami untuk informasi mengenai biaya tambahan ini. |
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| Penyimpanan | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Informasi keselamatan kerja | |||
|---|---|---|---|
| Frase S |
S: 26-36/37/39-45 |
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| Frase R |
R: 23/24/25-35-36/37/38-40-43 |
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| Data | |||
|---|---|---|---|
 Neutralization of bcl-2 positive samples using a bcl-2 monoclonal antibody (Cat. No. OP60).  The pooled coefficient of variation (according to the formula of Henry, et al., 1974) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  The pooled coefficient of variation (according to the formula of Henry, et al., 1975) is plotted against bcl-2 concentrations. The pooled data were collected from samples run (in replicates of four) on two separate occasions using two different lots of coated plates on each occasion (total of four assays). The triangles represent samples run on one occasion.  Human sera and plasma samples were spiked with bcl-2 protein to obtain a final concentration of 42 units/ml and analyzed as outlined above. The control represents bcl-2 spiked into sample diluent.  Dilution recovery from a bcl-2 positive cell line using different antigen extraction agents (different detergents).  Dilution recovery using different cell lines. |
