CBA003
InnoZyme™ Gelatinase (MMP-2/MMP-9) Activity Assay kit, Fluorogenic
MMP-2/MMP-9 Activity Assay Kit, Fluorogenic
Untuk pertanyaan umum, silakan hubungi Layanan Pelanggan kami:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
A sensitive fluorogenic assay (Exc. max: ~325 nm; Emi. max.: ~393 nm) for the measurement of gelatinases (MMP-2 and MMP-9). Also useful for the screening of gelatinase inhibitors.
| Informasi produk | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Fluorogenic | ||
| Protect from Light | Ya | ||
| Avoid freeze/thaw | Ya | ||
| Sensitivity | 20 ng/ml | ||
| Assay time | 2 - 6 h | ||
| Sample type | Cell lysate, culture supernatant, tissue extract, plasma, serum, purified enzyme | ||
| Positive control | MMP-9 (Cat. No. PF140) | ||
| Kit contains | Gelatinase Substrate, pro-MMP-2 Positive Control, Inhibitor, APMA, Assay Buffer, 96-Well Plate, Plate Sealer, and a user protocol | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
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Informasi Bahan Berbahaya: Karena sifat dari Bahan Berbahaya ini kami mengenakan biaya pengiriman tambahan yang dapat ditambahkan ke pesanan Anda. Kemasan tertentu mungkin saja tidak dikenakan tambahan biaya pengiriman bahan berbahaya. Silakan hubungi kami untuk informasi mengenai biaya tambahan ini. |
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| Penyimpanan | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 Human Pro-MMP-2 (supplied) was activated as outlined in the Reagent Preparation section above and assayed for activity as outlined in the Detailed Protocol, incubating for a total of 3 h.  Activated MMP-9 (Cat. No. PF140) was assayed without APMA at 37°C for 3 h according to the Detailed Protocol outlined above.  Serum from 5 different donors was diluted 1:5 in Activation Buffer and HT1080 culture supernatant was diluted 1:3 with Activation Buffer. All samples were incubated at 37°C for 3 h according to the Detailed Protocol outlined above. |
