CBA044  K-LISA™ IKKβ Inhibitor Screening Kit

Inhibitor of nuclear factor kappa B kinase beta subunit screening kit, I-kappa-B-kinase beta screening kit, NFKBIKB screening kit, Nuclear factor NF-kappa-B inhibitor kinase beta screening kit, I-kappa-B kinase 2 screening kit, IKK2 screening kit, IκBKB screening kit

The K-LISA™ IKKβ-Inhibitor Screening Kit is designed for rapid in vitro screening of IKKβ inhibitors. The assay is an ELISA-based activity assay that utilizes a 50-amino acid GST-IκBα fusion polypeptide substrate that includes the Ser³² and Ser³⁶ IKKβ phosphorylation sites. The GST-IκBα substrate and IKKβ are incubated in the presence of IKKβ inhibitors in the wells of a Glutathione-Coated 96-Well Plate, which allows for substrate phosphorylation and capture in a single step. The phosphorylated GST-IκBα substrate is detected using an Anti-Phospho IκBα (Ser³²/Ser³⁶) antibody, followed by the HRP-Conjugate and color development with TMB Substrate. ELISA Stop Solution is used to stop the color development and the absorbance is read at 450 nm preferably with a reference wavelength of 540-600 nm. The absorbance is directly related to the level of IKKβ activity.