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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of captured nucleosomes and is detected following incubation with streptavidin-HRP conjugate. HRP catalyzes the conversion of colorless tetramethylbenzidine to blue. The addition of stop solution changes the color to yellow, the intensity of which is proportional to the number of nucleosomes in the sample. By comparing the absorbance of unknown with that obtained from the standards, one can assign a nucleosome unit value to the unknown sample. Can be used to assign apoptotic index.
| Informasi produk | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | a broad range of species | ||
| Applications |
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| Protect from Moisture | Ya | ||
| Avoid freeze/thaw | Ya | ||
| Unit definition | One unit (U) is defined as the amount of nucleosomes from 444 UV-treated Daudi cells. | ||
| Sensitivity | 0.15 U/ml | ||
| Assay range | 00 - 12 U/ml | ||
| Assay time | 5 h | ||
| Sample type | Cell lysates | ||
| Kit contains | 96-Well Plate (12 removable strips) Coated with a DNA Binding Protein, Nucleosome Standard, Lysis Buffer/Sample Diluent, Biotinylated Anti-Histone 3 Antibody, 400X HRP-Streptavidin Conjugate, Conjugate Diluent, Chromogenic Substrate, 20X Concentrated Wash Solution, Stop Solution, 4 Plate Sealers, and a user protocol. | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
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Informasi Bahan Berbahaya: Karena sifat dari Bahan Berbahaya ini kami mengenakan biaya pengiriman tambahan yang dapat ditambahkan ke pesanan Anda. Kemasan tertentu mungkin saja tidak dikenakan tambahan biaya pengiriman bahan berbahaya. Silakan hubungi kami untuk informasi mengenai biaya tambahan ini. |
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| Penyimpanan | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Informasi keselamatan kerja | |||
|---|---|---|---|
| Frase S |
S: 26-36/37/39-45 |
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| Frase R |
R: 23/24/25-35-36/37/38-40-43 |
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