539742
PTP Assay Kit, Fluorometric
Protein Tyrosine Phosphatase
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Telefon: +49 6151 72-0
Faks: +49 6151 72 2000
31 May 2012
memuat
A fluorometric assay kit for the measurement of Protein Tyrosine Phosphatase (PTP) activity. The assay uses a fluorogenic phosphopeptide substrate quenched internally by resonance energy transfer. The substrate contains a fluorophore (MCA) and a quencher group (DNP) as well as a phosphotyrosine residue located between these groups. Reaction of the substrate with PTP followed by treatment of the dephosphorylated substrate with chymotrypsin results in the cleavage of the peptide, which results in an increase in fluorescence.
| Informasi produk | |||
|---|---|---|---|
| Format | 96-well format | ||
| Form | 96 Tests | ||
| Detection method | Fluorometric | ||
| Protect from Light | Ya | ||
| Sensitivity | 0.2-0.4 pmol | ||
| Assay time | 2 h | ||
| Sample type | Purified enzyme | ||
| Kit contains | PTP Substrate, Assay Buffer, Chymotrypsin Solution, Control Peptide, Microtiter Plate, Stop Solution, and a user protocol. | ||
| Simpan dan kirim informasi | |||
|---|---|---|---|
| Penyimpanan | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
||
| Informasi keselamatan kerja | |||
|---|---|---|---|
| Frase S |
S: 23-24-26-36/37 |
||
| Frase R |
R: 36/37/38-42 |
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