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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
Note: 1 kit = 40 assays.
| 製品情報 | |||
|---|---|---|---|
| Format | Immunoblot | ||
| Form | 40 Tests | ||
| Detection method | Immunoblot | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | はい | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates, tissue extracts | ||
| Kit contains | Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3α Protein/ATP Mixture, Kinase Assay Buffer, GSK-3α, Phospho-Specific Antibody, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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