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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
This product has been discontinued.
We are offering Glutathione Peroxidase Assay Kit (Cat. No. 353919) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.
A spectrophotometric assay kit based on the method described by Paglia and Valentine. Glutathione peroxidase (GPx) catalyzes the reduction of an organic peroxide while forming oxidized glutathione (GSSG). GSSG is reduced to GSH by the enzyme glutathione reductase, and NADPH is oxidized to NADP in the catalytic cycle. GPx activity is quantitated by measuring the change in absorbance at 340 nm caused by the oxidation of NADPH. Each kit can be used to perform up to 100 assays.
| 製品情報 | |||
|---|---|---|---|
| Format | Cuvette | ||
| Form | 100 Tests | ||
| Detection method | Colorimetric | ||
| Unit definition | One unit is defined as the amount of enzyme that will cause the oxidation of 1.0 μM of NADPH to NADP per minute at 37°C measured at 340 nm. | ||
| Assay range | 5.6-24 mU/ml | ||
| Assay time | 2 h | ||
| Sample type | Cell lysates, tissue extracts, erythrocyte lysates | ||
| Kit contains | Glutathione, glutathione reductase, NADPH, t-butylhydroperoxide, buffer, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
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危険物に関する注意： 今回の納品における危険物の特性により、追加の送料が適用される場合があります。サイズによって危険物にかかる追加送料が免除されることもあります。送料に関する詳細はオーダーマネージメントまでお問い合わせください。 |
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| Storage | Multiple Storage Requirements | ||
| Ship |
Multiple Storage Conditions
Multiple Toxicity Values, refer to MSDS |
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| 安全性情報 | |||
|---|---|---|---|
| S フレーズ |
S: 23-36/37/39-16-17 |
||
| R フレーズ |
R: 8-20/21/22-34-40-44 |
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| データ | |||
|---|---|---|---|
 Cellular Glutathione Peroxidase at a 288 mU/ml assayed at 23°C. |
