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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
The K-LISA™ Checkpoint Activity Kit is a rapid, sensitive, ELISA-based activity assay suitable for measuring the kinase activity of purified or partially purified Chk1 and Chk2 preparations, in vitro Chk1 and Chk2 inhibitor screening, and for assessing the regulation of Chk1 and Chk2 in cell signaling. The assay utilizes a biotinylated peptide substrate (KKKVSRSGLYRSPSMPENLNRPR) that is phosphorylated on the third serine by Chk1 and Chk2. Biotinylated Chk substrate and sample containing Chk1 or Chk2 are incubated in the presence of ATP in wells of the streptavidin-coated 96-well plate, which allows for phosphorylation and substrate capture in a single step.
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
| 代替品 | |
|---|---|
| CBA020 | K-LISA™ Checkpoint Activity Kit |
| 関連製品 K-LISA™ Checkpoint Activity Kit | |
| CBA020 | K-LISA™ Checkpoint Activity Kit |
| アクセサリー/ K-LISA™ Checkpoint Activity Kit |
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Avoid freeze/thaw | はい | ||
| Sensitivity | 25 pg (0.018 mU) purified Chk1 | ||
| Assay range | 25-725 µU (Chk1) and 260 µU - 26 mU (Chk2) | ||
| Assay time | 3 h | ||
| Sample type | Cell lysates, tissue extracts, purified enzymes | ||
| Kit contains | Biotinylated Chk1 Substrate, Phosphoserine Detector Antibody, Streptavidin Coated 96-Well Plate Strips, HRP-Conjugated Secondary Antibody, Serine/Threonine Kinase Assay Buffer, ATP/MgCl2, Chk1 Inhibitor, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. Purified Chk1 is available separately (Cat. No. 220479). | ||
| 保管と送付情報 | |||
|---|---|---|---|
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危険物に関する注意： 今回の納品における危険物の特性により、追加の送料が適用される場合があります。サイズによって危険物にかかる追加送料が免除されることもあります。送料に関する詳細はオーダーマネージメントまでお問い合わせください。 |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| データ | |||
|---|---|---|---|
 The activity of His<b>•</b>Tag<sup>®</sup>, Human, Recombinant Chk1 (Cat. No. 220479) (17-2200 pg) was determined using protocol A as indicated in the Detailed Protocol section. Assay range: 34 pg-1100 pg (740 Units/mg).  The activity of Human, Recombinant Chk2 (0.06-60 ng) was determined using protocol A in the Detailed Protocol section. Assay range: 200 pg-20 ng (1283 Units/mg). |
