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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
The K-LISA™ PTK Screening Kit, an ELISA-based protein tyrosine kinase (PTK) activity assay, is a rapid, non-radioactive method to screen for PTK activity of purified enzyme samples as well as in cell lysates or subcellular fractions and tissue extracts. This colorimetric assay can detect activity at picomolar concentrations with purified PTKs and in as little as 5 µg total protein from cell lysates and 20 ng total protein from tissue extracts. The kit uses three different random polypeptide substrates (EAY, E4Y, or EY) that are composed of different molar ratios of glutamic acid (E), alanine (A), and tyrosine (Y), (1:1:1, 4:1, and 1:1, respectively) and are immobilized on two 96-well plates. To study general PTK activity, substrate selectivity, or changes in PTK activity associated with inhibitor or mutational effects by either comparative or ratiometric analysis.
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 216 Tests, 72 Controls | ||
| Detection method | Colorimetric | ||
| Protect from Moisture | はい | ||
| Assay time | 3 h | ||
| Sample type | Cell lysates, tissue extracts, purified enzyme | ||
| Kit contains | Two 96-well reaction plates, reaction buffer, protease inhibitor cocktail, DTT, activated sodium orthovanadate, ATP, stop solution, Peroxidase conjugated Anti-phosphotyrosine, TMB, PBST tablets, positive control, plate sealers, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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