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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of captured nucleosomes and is detected following incubation with streptavidin-HRP conjugate. HRP catalyzes the conversion of colorless tetramethylbenzidine to blue. The addition of stop solution changes the color to yellow, the intensity of which is proportional to the number of nucleosomes in the sample. By comparing the absorbance of unknown with that obtained from the standards, one can assign a nucleosome unit value to the unknown sample. Can be used to assign apoptotic index.
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | a broad range of species | ||
| Applications |
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| Protect from Moisture | はい | ||
| Avoid freeze/thaw | はい | ||
| Unit definition | One unit (U) is defined as the amount of nucleosomes from 444 UV-treated Daudi cells. | ||
| Sensitivity | 0.15 U/ml | ||
| Assay range | 00 - 12 U/ml | ||
| Assay time | 5 h | ||
| Sample type | Cell lysates | ||
| Kit contains | 96-Well Plate (12 removable strips) Coated with a DNA Binding Protein, Nucleosome Standard, Lysis Buffer/Sample Diluent, Biotinylated Anti-Histone 3 Antibody, 400X HRP-Streptavidin Conjugate, Conjugate Diluent, Chromogenic Substrate, 20X Concentrated Wash Solution, Stop Solution, 4 Plate Sealers, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
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危険物に関する注意： 今回の納品における危険物の特性により、追加の送料が適用される場合があります。サイズによって危険物にかかる追加送料が免除されることもあります。送料に関する詳細はオーダーマネージメントまでお問い合わせください。 |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| 安全性情報 | |||
|---|---|---|---|
| S フレーズ |
S: 26-36/37/39-45 |
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| R フレーズ |
R: 23/24/25-35-36/37/38-40-43 |
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