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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
Fast, sensitive assay for the in vitro quantitation of PCNA protein. The kit has broad reactivity, and is suitable to detect PCNA in autoimmune sera samples. The assay was validated using the BrdU Cell Proliferation Assay (Cat. No. QIA58).
Proliferating cell nuclear antigen (PCNA) has no known enzymatic activity; however, it can interact with a variety of proteins and regulate their activities. These varied interactions with DNA metabolic proteins imply that PCNA is a central factor for the coordination of DNA replication, DNA repair, epigenetic inheritance, and cell-cycle control.
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well strip plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Do not freeze | はい | ||
| Unit definition | One unit (U) is defined as the amount of PCNA measured from ~ 2.6 x 103 SW620 cells. | ||
| Specificity | Human PCNA protein | ||
| Assay range | 3.125-200 ng/ml of PCNA | ||
| Assay time | 3.5 h | ||
| Sample type | Cell lysates, tissue culture media , serum, or plasma | ||
| Kit contains | 96-Well Removable Strip Plate, 2 vials of PCNA Standard, Detector Antibody, 400X Conjugate, Conjugate Diluent, Substrate, Sample Diluent, 20X Plate Wash Concentrate, Antigen Extraction Agent, Stop Solution, Plate Sealers, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
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危険物に関する注意： 今回の納品における危険物の特性により、追加の送料が適用される場合があります。サイズによって危険物にかかる追加送料が免除されることもあります。送料に関する詳細はオーダーマネージメントまでお問い合わせください。 |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| 安全性情報 | |||
|---|---|---|---|
| S フレーズ |
S: 26-36/37/39-45 |
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| R フレーズ |
R: 23/24/25-35-36/37/38-40-43 |
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| データ | |||
|---|---|---|---|
 The mean signal of each standard run in replicates of two in four assays using two different batches of plates and two different lots of detector antibody. <b>Note:</b> 44 units/ml of the old standard (lysate) corresponds to about 200 ng/ml of the new standard. |
