CBA004
PhosphoDetect™ Akt (pThr308) ELISA Kit
Protein Kinase B, Phospho-Specific (Thr308) ELISA Kit
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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
Detects Akt when phosphorylated on Thr308. Akt is activated by a variety of stimuli including insulin through phosphorylation on Thr308 and Ser473. Once phosphorylated Akt migrates to the nucleus where it is involved in a variety of cellular processes such as the transduction of signals that prevent apoptosis. Although this kit was developed for human samples, it has been found to cross react with mouse and rat.
| 代替品 | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| 関連製品 PhosphoDetect™ Akt (pThr308) ELISA Kit | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| アクセサリー/ PhosphoDetect™ Akt (pThr308) ELISA Kit |
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | はい | ||
| Sensitivity | ≤0.8 units/ml | ||
| Assay range | 1.6-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Positive control | Akt (pThr308) | ||
| Kit contains | Coated 96-Well Plate, Akt Phospho-Thr308 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| データ | |||
|---|---|---|---|
 The data presented in figure 1 shows that the sensitivity of the ELISA is approximately the same as that of immunoblotting. The bands shown in the immunoblot were developed using Anti-Akt, Phospho-Specific (Thr<sup>308</sup>), (Cat. No. 124001), an alkaline phosphatase conjugated anti-rabbit IgG analyzed by chemiluminescent substrate, and autoradiography. |
