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メルク株式会社
目黒区下目黒1-8-1
153-8927 東京
Japan
TEL： 0120-189-390
Fax: 0120-189-350
E-mail mj_service@merckgroup.com
31 5月 2012
ローディング
Detects p53 when phosphorylated on Ser15 in human cell lysates. Does not react with mouse or rat. p53 is phosphorylated on Ser15 by ATM, ATR, and DNA protein kinase leading to reduced interactions with its negative regulator, MDM2, and accumulation of p53.
| 代替品 | |
|---|---|
| 71296 | PhosphoSafe™ Extraction Reagent |
| 関連製品 PhosphoDetect™ p53 (pSer¹5) ELISA Kit | |
| 71296 | PhosphoSafe™ Extraction Reagent |
| アクセサリー/ PhosphoDetect™ p53 (pSer¹5) ELISA Kit |
| 製品情報 | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Species reactivity | human | ||
| Avoid freeze/thaw | はい | ||
| Unit definition | One unit is defined as the amount of p53 (pSer¹5) derived from approximately 11,000 CCRF/CEM cells treated with 5 nM actinomycin D for 20 hours. | ||
| Sensitivity | ≤0.9 units/ml | ||
| Assay range | 1-100 units/ml | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates | ||
| Kit contains | Coated 96-Well Plate, p53 Phospho-Ser¹5 Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Covers, and a user protocol. | ||
| 保管と送付情報 | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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