For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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This kit is designed to measure human 20S proteasome levels in biological samples such as cell lysates and biological fluids like human plasma.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 tests | ||
| Avoid freeze/thaw | Yes | ||
| Assay range | 3-200 ng/ml as measured with purified, human 20S Proteasome. | ||
| Assay time | 3.5 hours | ||
| Sample type | cell lysates and human plasma | ||
| Store and ship information | |||
|---|---|---|---|
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 24/25-26-36/37/39-45-A09 Avoid contact with skin and eyes. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). |
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| R Phrase |
R: 20/21/22-25-35-36/37/38-43-61 Harmful by inhalation, in contact with skin and if swallowed. Toxic if swallowed. Causes severe burns. Irritating to eyes, respiratory system and skin. May cause sensitization by skin contact. May cause harm to the unborn child. |
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| Data | |||
|---|---|---|---|
  Human 20S proteasome was measured as outlined in the Detailed Protocol. Absorbance was measured at dual wavelengths, 450 nm/595 nm using an ELISA plate reader (Model: V<sub>max</sub> kinetic microplate reader, Molecular Devices).  20S proteasome was measured as outlined in the Detailed Protocol using plasma samples from healthy donors, prepared with citrate as anticoagulant, and obtained from the San Diego Blood Bank. Samples were initially diluted 10-fold prior to carrying out the assay. Absorbance was measured at dual wavelengths, 450 nm/595 nm using an ELISA plate reader (Model: V<sub>max</sub> kinetic microplate reader, Molecular Devices).  Cells were cultured in DMEM with 10% FCS and harvested at 80-90% confluency. Total cell lysates were prepared using CytoBuster™ Protein Extraction Reagent (Cat. No. 71009) according to the manufacturer's recommended protocol. Protein concentration in cell lysates was determined using a BCA protein assay. The concentration of 20S proteasome was measured as outlined in the Detailed Protocol. Absorbance was measured at dual wavelengths, 450 nm/595 nm using an ELISA plate reader (Model: V<sub>max</sub> kinetic microplate reader, Molecular Devices). The amount of 20S proteasome (in µg) in human cell lysates was calculated per mg total protein. |
