PF124
ADAM10, His•Tag®, Mouse, Recombinant, S. frugiperda
Kuzbanian Metalloproteinase
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Recombinant, mouse ADAM10 (mature, active mouse ADAM10 ectodomain; amino acids 19-673) fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. An integral membrane proteinase involved in notch signaling. Also considered as an α-secretase candidate.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Formulation | Lyophilized from a filtered solution of 100 mM NaCl, 25 mM MES, 10 µM ZnCl2, 0.02% BRIJ®-35 Detergent, pH 5.5. | ||
| Molar mass | 60000 | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥5 pmol/min/µg | ||
| Activity | ≥5 pmol/min/µg as measured by the cleavage of 10 µM of the fluorescent substrate MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2 (Cat. No. 616402) by 100 ng of the enzyme in 100 µl of reaction buffer at 37°C. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Contaminants | Endotoxin: ≤1 EU/µg ADAM10 | ||
| Solubility | 25 mM Tris-HCl buffer, pH 7.5 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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