PF113
ADAMTS4, Truncated, His•Tag®, Human, Recombinant, Insect Cells
Aggrecanase 1
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Recombinant, human, truncated ADAMTS4 (amino acids 213-579) fused at the C-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression. ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motif-4) belongs to a novel family of extracellular proteinases that hydrolyze aggrecan, the major proteoglycan of articular cartilage. Useful for the study of extracellular proteoglycan degradation, screening for inhibitors of proteoglycan hydrolysis, and as a standard in enzymatic and immunochemical assays.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 0.05% BRIJ® 35 Detergent, pH 7.5. | ||
| Preservative | None | ||
| Molar mass | 42000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥1.5 U/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme necessary to hydrolyze 1.0 nmol of recombinant aggrecan interglobular domain (Cat. No. PF114) per min at 37°C, pH 7.5. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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