For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
loading
Useful for the non-radioactive assay of Akt activity in cell lysate or tissue extract lysates. Akt is first enriched via immunoprecipitation with an anti-Akt antibody and then tested for its ability to phosphorylate GSK-3α, an Akt substrate. Phosphorylated GSK-3α is detected through immunoblotting with anti-GSK-3α phospho-specific antibody. Physiologically, GSK-3α is inactivated by Akt phosphorylation. Akt is activated by diverse stimuli such as growth factors and regulates many cellular processes such as apoptosis, metabolism, and proliferation through interactions with a variety of substrates.
Note: 1 kit = 40 assays.
| Product information | |||
|---|---|---|---|
| Format | Immunoblot | ||
| Form | 40 Tests | ||
| Detection method | Immunoblot | ||
| Species reactivity | human, mouse, rat | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 4 h | ||
| Sample type | Cell lysates, tissue extracts | ||
| Kit contains | Kinase Extraction Buffer, Akt Antibody, Protein A Agarose, GSK-3α Protein/ATP Mixture, Kinase Assay Buffer, GSK-3α, Phospho-Specific Antibody, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
||
