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31 May 2012
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Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
| Product information | |||
|---|---|---|---|
| Form | White crystalline suspension | ||
| Formulation | Crystalline suspension in 3.2 M (NH4)2SO4, 10 mM MgCl2, 10 mM Tris-HCl, pH 7.5. | ||
| Molar mass | 280000 | ||
| Specific activity | ≥10 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2. | ||
| Activity | ≥50 units/ml | ||
| Contaminants | Oxidases: ≤3% | ||
| pH value | optimum 7.0 - 7.5 | ||
| Solubility | H2O or physiological buffers | ||
| EC number | 3.4.11.2 | ||
| CAS number | 9054-63-1 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Ambient Temperature Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-26-36 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
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| R Phrase |
R: 36/37/38-42 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation. |
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