For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
01 June 2012
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A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to biotin or to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
| Substitutes | |
|---|---|
| PF036 | Annexin V-Biotin Apoptosis Detection Kit |
| Substitutes for Annexin V-Biotin Apoptosis Detection Kit II | |
| PF036 | Annexin V-Biotin Apoptosis Detection Kit |
| Product information | |||
|---|---|---|---|
| Format | Flow cytometry or fluorescence microscopy | ||
| Form | 20 Tests | ||
| Detection method | Fluorescence | ||
| Applications |
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| Assay time | 1 h | ||
| Sample type | Intact cells | ||
| Kit contains | Annexin V-Biotin, Binding Buffer, Propidium Iodide and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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