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01 June 2012
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Recognizes the ~100 kDa AHR protein in COLO 320 HSR and HeLa cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids of 721-821 human AHR, expressed as a GST fusion protein | ||
| Clone | 3B12 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | COLO 320 HSR cells, HeLa cells | ||
| Negative control | 293T | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human AHR by immunoblotting. Sample: Whole cell lysate from COLO 320 HSR cells. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human AHR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AHR Mouse mAb (3B12) (Cat. No. DR1090) (10 µg/ml). Detection: fluorescence. |
