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01 June 2012
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Recognizes the ~35-37 kDa AURKB protein in HepG2 and HeLa cells and human small intestine tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids of 1-90 human AURKB, expressed as a GST fusion protein | ||
| Clone | 6A6 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
|
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| Avoid freeze/thaw | Yes | ||
| Positive control | HepG2 cells, HeLa cells, Human small intestine tissue | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human AURKB by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AURKB Mouse mAb (6A6) (Cat. No. ST1644) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human AURKB by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-AURKB Mouse mAb (6A6) (Cat. No. ST1644) (12 µg/ml). Detection: fluorescence.  Detection of human AURKB by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-AURKB Mouse mAb (6A6) (Cat. No. ST1644) (4 µg/ml). Detection: colorimetric (DAB). |
