For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
loading
Recognizes the ~110 kDa Notch 1 protein cleaved at (Val1744) in differentiated 3T3L1 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids surrounding the Val¹744 cleavage site of human Notch 1 | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human, monkey, mouse, rat | ||
| Preservative | None | ||
| Applications |
|
||
| Avoid freeze/thaw | Yes | ||
| Positive control | Differentiated 3T3-L1 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Data | |||
|---|---|---|---|
 Detection of mouse cleaved Notch 1 (Val<sup>1744</sup>) by immunoblotting. Samples: Whole cell lysate from undifferentiated 3T3-L1 cells (lane 1) or 3T3-L1 cells differentiated for 10 days (lane 2). Primary antibody: Anti-Cleaved Notch 1 (Val<sup>1744</sup>) Rabbit pAb (Cat. No. ST1028) (1:1000). Detection: chemiluminescence. |
