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31 May 2012
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Recognizes the ~19 kDa p19ARF protein in CTLL2 cells and mouse embryo fibroblasts.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids 54-75 of mouse p19ARF, conjugated to KLH | ||
| Form | Liquid | ||
| Formulation | In PBS, 1% BSA. | ||
| Species reactivity | mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Mouse embryo fibroblasts or CTLL2 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of mouse p19ARF by immunoblotting. Sample: Whole cell lysate from wild-type mouse embryonic fibroblasts (lanes 1 and 2) and whole cell lysate from mouse embtyonic fibroblasts bearing a p19ARF gene knockout as a negative control (lane 3). Primary antibody: Anti-p19ARF (54-75) (Ab-1) Rabbit pAb (Cat. No. PC435) (1 µg/ml). Detection: chemiluminescence. |
