Information
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DR1028  Anti-PARC/H7-AP1 (900-950) Rabbit pAb

Anti-p53 Associated Parkin-Like Cytoplasmic Protein, Anti-UbcH7 Associated Protein 1

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18 April 2014

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Recognizes the ~270 kDa PARC/H7-AP1 protein in U20S cells.
Product information
Host Rabbit
Isotype IgG
Immunogen a synthetic peptide corresponding to amino acids 900-950 of human PARC
Form Liquid
Formulation In Tris-citrate/phosphate buffer, pH 7.0-8.0.
Species reactivity human, not mouse
Preservative ≤0.1% sodium azide
Applications
Immunoblotting (Western Blotting)
Immunoprecipitation
Positive control U2OS cells
Store and ship information
Storage +2°C to +8°C
Ship Blue Ice Only
Highly Toxic
Data

Lanes 1, 2: Detection of human PARC/H7-AP1 by immunoblotting (Lanes 1 and 2). Samples: Whole cell lysate (40 µg) from untreated U2OS cells (Lane 1) or U2OS cells that stably express a short hairpin RNA (shPARC) against PARC/H7-AP1 (Lane 2). Primary antibody: Anti-PARC/H7-AP1 (900-950) Rabbit pAb (Cat. No. DR1028) (1:2500). Detection: chemiluminescence. Lanes 3, 4: Detection of human PARC/H7-AP1 by immunoprecipitation followed by immunoblotting. Samples: Whole cell lysate (500 µg) from untreated U2OS cells. Antibodies for IP: Normal rabbit IgG (2 µg/mg lysate) (Lane 3) and Anti-PARC/H7-AP1 (Cat. No. DR1028) (2 µg/mg lysate) (Lane 4). Immunoblotting conditions: same as Lanes 1 and 2.

© Merck KGaA, Darmstadt, Germany, 2014


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