AP1041
Anti-PTEN Mouse mAb (EMD-15E10)
Anti-MMAC, Anti-Phosphatase and Tensin Homologue Deleted on Chromosome Ten, Anti-TEP-1
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Recognizes the ~55 kDa PTEN protein in MCF-7 cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | full-length, recombinant human PTEN expressed in Sf9 cells | ||
| Clone | EMD-15E10 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | ≤ 0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | MCF7 cells | ||
| Negative control | MDA-MB-468 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Highly Toxic |
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| Data | |||
|---|---|---|---|
 Detection of human PTEN by immunoprecipitation. Samples: Whole cell lysate (125 µg) from MDA-MB-468 cells (Lane 1) and MCF7 cells (Lane 2). Antibody for IP: Anti-PTEN Mouse mAb (EMD-15E10) (Cat. No. AP1041) (2 µg). Antibody for immunoblotting: Anti-PTEN Rabbit pAb. Detection: colorimetric. |
