70664
Benzonase® Nuclease, Purity > 99%
Effective viscosity reduction and removal of nucleic acids from protein solutions
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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Benzonase® Nuclease is a genetically
engineered endonuclease from Serratia
marcescens. It degrades all forms of DNA and
RNA (single stranded, double stranded, linear
and circular) while having no proteolytic
activity. It is effective over a wide range of
conditions and possesses an exceptionally
high specific activity. The enzyme completely
digests nucleic acids to 5′-monophosphate
terminated oligonucleotides 2 to 5 bases in
length (below the hybridization limit), which
is ideal for removal of nucleic acids from
recombinant proteins, enabling compliance
with FDA guidelines for nucleic acid
contamination. The ability of Benzonase to
rapidly hydrolyze nucleic acids makes the
enzyme an excellent choice for viscosity
reduction to reduce processing time and
increase yields of protein. For example, the
enzyme is compatible with BugBuster® and
PopCulture® Protein Extraction Reagents
and can therefore be added along with these
reagents to eliminate viscosity and remove
nucleic acids from E. coli extracts.
The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.
Total endotoxin: < 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.
The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42°C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/µl and at a high concentration (HC) of 250 U/µl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 µm filtered solution in 50% glycerol. Store at -20°C.
Total endotoxin: < 0.25 EU/1,000 units. Purity: > 99% by SDS-PAGE.| Product information | |||
|---|---|---|---|
| Unit definition | One unit is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA. | ||
| Purity | > 99% by SDS-PAGE | ||
| Contaminants | Total endotoxin: < 0.25 EU/1000 units. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
