565758
β-Secretase Substrate IV, Fluorogenic
FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
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Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
| Product information | |||
|---|---|---|---|
| Peptide Sequence | H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH | ||
| Form | Red lyophilized solid | ||
| Formulation | Supplied as a trifluoroacetate salt. | ||
| Molar mass | 2005.2 | ||
| Protect from Light | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥95% by HPLC | ||
| Excitation max. | ~350 nm | ||
| Emission max. | ~490 nm | ||
| Solubility | DMSO | ||
| Chemical formula | C91H129N25O25S | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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