565759
β-Secretase Substrate V, Fluorogenic
FS-2, MCA-SEVNLDAEFK(DNP)-CONH2, Memapsin 2 Substrate II, Fluorogenic
For general questions please contact our Customer Service:
Merck KGaA
Frankfurter Str. 250
64293 Darmstadt
Germany
Phone: +49 6151 72-0
Fax: +49 6151 72 2000
31 May 2012
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A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (at the Leu-Asp bond) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 and Gly45p-memapsin 2 are 0.056, 0.080 and 0.160, respectively.
| Product information | |||
|---|---|---|---|
| Peptide Sequence | MCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DNP)-CONH2 | ||
| Form | Off-white solid | ||
| Molar mass | 1532.5 | ||
| Protect from Light | Yes | ||
| Packaged under inert gas | Yes | ||
| Purity | ≥95% by HPLC | ||
| Excitation max. | ~328 nm | ||
| Emission max. | ~393 nm | ||
| Solubility | DMSO | ||
| Chemical formula | C68H89N15O26 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Standard Handling |
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