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31 May 2012
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Detects and quantifies the levels of c-Met protein independent of its phosphorylation state. The ligand for c-Met, HGF/SF, is a member of the plasminogen-related growth factor family, which is synthesized as an inactive pro-form. HGF/SF stimulation of c-Met expressing cells enhances proliferation, migration, morphogenesis, and protease synthesis, characteristics that are associated with invasive cell phenotype. This kit is designed for use with human samples.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Species reactivity | human | ||
| Sensitivity | <4 ng/ml | ||
| Assay range | 0.78-50 ng/ml | ||
| Assay time | 4 h | ||
| Sample type | Cells | ||
| Kit contains | Coated 96-Well Plate, c-Met Standard, Diluents, Detector Antibody, Secondary Antibody, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Met. The data presented in Figure 1 show that the sensitivity of the ELISA is ~2x greater than that of immunoblotting. The bands shown in the immunoblot data were developed using an anti-c-Met antibody and chemiluminescent detection. |
