208782  Calmodulin-SH, Wheat Germ

CaM

This product has been discontinued.

Calmodulin (CaM) purified from wheat germ (Triticum aestivum). Wheat germ calmodulin is unique in that it contains cysteine at position 26 (Cys²⁶), which is in contrast to mammalian CaM that does not possess this residue. The single SH moiety on Cys²⁶ makes this CaM preparation ideal for applications requiring both calmodulin activation and residue specific tagging. The sulfhydryl can be labeled using SH-reactive protein-modification reagents like fluorescent maleimide derivatives. Additionally, wheat germ CaM contains only a single tyrosine residue (Tyr¹³⁸), compared to mammalian CaM that has two tyrosine residues, which can serve as a monospecific C-terminal labeling site. Using Cys²⁶ and Tyr¹³⁸ allow wheat germ CaM to be independently derivatized at both the N- and C-termini making it a useful tool for structural, proteomic biomarker discovery and protein-protein interaction studies. Wheat germ CaM also contains no tryptophan residues, resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.